Co-fractionation of an endonuclease activity during the purification of DNA polymerase-alpha from regenerating rat liver. Properties and separation from DNA polymerase.
Eur J Biochem, 1975/10/15;58(2):461-6.
PMID: 241642
Abstract
The presence of endonuclease activity associated with DNA polymerase was detected during the purification of high-molecular-weight DNA polymerase-alpha from regenerating rat liver by the use of a highly sensitive test. This endonuclease activity co-fractionated with DNA polymerase in a great variety of purification procedures involving ion-exchange chromatographies or molecular weight fractionation, but was further completely separated from DNA polymerase activity by using affinity chromatography on DNA-cellulose. The endonuclease acted on native or denatured DNA by introducing single-strand nicks in the DNA molecules; its enzymatic properties indicate that it could act in polymerisation conditions in vitro.
MeSH terms
Animals; Calcium; Chromatography; DNA Nucleotidyltransferases; DNA, Viral; Endonucleases; Hydrogen-Ion Concentration; Hydroxymercuribenzoates; Liver; Liver Regeneration; Magnesium; Molecular Weight; Rats; Simian virus 40; Sodium
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