Antigenic comparison of swine influenza virus isolates.
Arch Virol, 1975;48(3):245-52.
Nath DM, Rodkey LS, Minocha HC
PMID: 241310
Impact factor: 2.685
Abstract
Cross hemagglutination inhibition (HI) and neuraminidase inhibition (NI) tests demonstrated strong antigenic relationship between A/Swine/Wisconsin/1/73 (SW/73) and A/Swine/Shope/15/31 (SW/31) influenza viruses. An eigthyone fold purification of virus was achieved by adsorption and elution followed by differential ultracentrifugation and sedimentation through linear sucrose gradient. Radioimmunoassay using purified 125I labeled viral antigens revealed antigenic variation between the two virus isolates. Neuraminidase of both viruses had pH optima between 6.5 and 7.0, and SW/31 enzyme was relatively more heat stable than SW/73.
MeSH terms
Antigens, Viral; Centrifugation, Density Gradient; Cross Reactions; Hemagglutination Inhibition Tests; Hemagglutinins, Viral; Hot Temperature; Hydrogen-Ion Concentration; Influenza A virus; Neuraminidase; Orthomyxoviridae; Radioimmunoassay; Ultracentrifugation; United States
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