[Microcalorimetric determination of thermochemical parameters of the phosphofructokinase reaction].
Acta Biol Med Ger, 1975;34(1):15-20.
Böhme HJ, Schellenberger W, Hofmann E
PMID: 241184
Abstract
A calorimetric procedure for determining deltaH, deltaG, deltaS and Keq of a bimolecular reaction with two or more products is described. By using this method the thermodynamic parameters of the phosphofructokinase reaction are determined. At pH 7.0 and 25 degrees C a reaction enthalpy of-6.96kcal/mole was found after correction for the neutralization enthalpy of the buffer and of the enthalpy difference of the magnesium complexes of ATP and ADP, respectively. The free energy of the phosphofructokinase reaction has been found under these conditions to be -3.96kcal/mole.
MeSH terms
Adenosine Diphosphate; Adenosine Triphosphate; Buffers; Calorimetry; Hydrogen-Ion Concentration; Magnesium; Microchemistry; Neutralization Tests; Phosphofructokinase-1; Thermodynamics
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