The differentiation of N-oxidation and N-dealkylation of N-ethyl-N-methylaniline by rabbit liver microsomes as distinct metabolic routes.
Xenobiotica, 1975/8;5(8):465-74.
Gorrod JW, Temple DJ, Beckett AH
PMID: 241156
Impact factor: 1.997
Abstract
1. N-Oxidation and N-dealkylation were shown to be separate routes of metabolism for N-ethyl-N-methylaniline. 2. N-Dealkylation, but not N-oxidation, is enhanced when NADH is present in addition to the NADPH-regenerating cofactors. 3. Some inhibitors, e.g. p-chloromercuribenzoate, inhibit N-dealkylation to a very much greater extent than they do N-oxidation. 4. N-Dealkylation is affected more by 'ageing' of the microsomal preparation or by the presence of bile salts, than is N-oxidation. 5. N-Oxidation followed by N-O-alpha-carbon migration of oxygen is only implicated to a very minor extent in the N-dealkylation process.
MeSH terms
2,2'-Dipyridyl; Acetone; Aniline Compounds; Animals; Chloromercuribenzoates; Cysteamine; Cytochrome P-450 Enzyme System; Dealkylation; Dithiothreitol; Ethylmaleimide; Kinetics; Liver; Male; Metyrapone; Microsomes, Liver; NADP; Oxidoreductases; Proadifen; Rats; Subcellular Fractions
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