Purification, properties and glycoprotein nature of arylsulfatase A from sheep brain.
Biochim Biophys Acta, 1975/9/22;403(1):113-21.
Balasubramanian KA, Bachhawat BK
PMID: 240423
Abstract
A simple and rapid method for the purification of arylsulfatase A (EC 3.1.6.1) from sheep brain has been developed. This includes the concanavalin A-Sepharose affinity chromatography and the pH-dependent polymerization and depolymerization of the enzyme. By these methods a homogeneous enzyme was obtained and the enzyme was purified 7180-fold. Sheep brain arylsulfatase A has been shown to be a glycoprotein containing 25% neutral sugar and 0.5% sialic acid. The constituent neutral sugars were identified as glucose and mannose.
MeSH terms
Animals; Arylsulfatases; Brain; Cerebroside-Sulfatase; Chromatography, Affinity; Concanavalin A; Glycoproteins; Hexoses; Molecular Weight; Sheep; Sialic Acids; Sulfatases
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