Chemostat culture of Escherichia coli K-12 limited by the activity of alkaline phosphatase.
Appl Microbiol, 1975/8;30(2):267-70.
PMID: 240310
Abstract
The growth-limiting reaction of a chemostat culture of Escherichia coli K-12 was the hydrolysis of beta-glycerophosphate by alkaline phosphatase. The culture was buffered at pH 5.2 where alkaline phosphatase was unable to supply phosphate to the cell at a rate sufficient to sustain the maximum rate of growth. Alkaline phosphatase activity in this system is discussed in terms of the so-called Flip-Flop mechanism.
MeSH terms
Alkaline Phosphatase; Escherichia coli; Glycerophosphates; Hydrogen-Ion Concentration; Kinetics; Mutation
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