Renin measurement in blood collected from the efferent arteriole of the kidney of the rat.
Pflugers Arch, 1975;356(3):277-86.
Johnston CI, Matthews PG, Davis JM, Morgan T
PMID: 239386
Impact factor: 4.458
Abstract
Plasma renin could be detected in 16 nl of rat plasma by incubating at 50 degrees C, pH 6.2 with 20 mul of a rat renin substrate. In 60 nl samples it could be estimated and differences in concentration of 20% could be detected. Plasma renin concentration was measured in blood from the superficial efferent arterioles of a rat's kidney. The concentration of renin in different efferent arterioles of the same rat were similar. The renin concentration of efferent arteriolar blood was 361 plus or (-1)89 (S.D.) ng Al ml minus 1 (blood) hour (-1) which was not different from the renin concentration of simultaneously taken femoral artery blood (340 +/- 217). Plasma concentration can be measured in efferent arteriolar blood and will allow the control of renin secretion to be studied.
MeSH terms
Angiotensin II; Animals; Arteries; Blood; Feedback; Femoral Artery; Hydrogen-Ion Concentration; Kidney Glomerulus; Rats; Renin
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