Fractionation of carrier ampholytes for isoelectric focusing.
J Chromatogr, 1975/6/18;109(2):357-64.
Gianazza E, Pagani M, Luzzana M, Righetti PG
PMID: 239019
Abstract
A simple method for fractionating synthetic carrier ampholytes is reported, based on the principle of continuous-flow isoelectric focusing in gel-stabilized layers. An 8% ampholyte solution, encompassing the pH range 3-9.5, is separated into 12 fractions in a chamber filled with Sephadex G-100 by a continuous-flow technique. We are thus able to obtain ampholytes of narrow pH range, encompassing approximately 2 pH units, whose resolving power is comparable with that obtained with commercial Ampholine covering similar pH ranges.
MeSH terms
Animals; Cattle; Chickens; Chymosin; Electrophoresis, Polyacrylamide Gel; Hydrogen-Ion Concentration; Isoelectric Focusing; Mucor; Pepsin A; Swine
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