Synthesis of polyglycerol phosphate by Bacillus stearothermophilus.
J Biochem, 1975/3;77(3):579-86.
PMID: 238960
Impact factor: 3.241
Abstract
A particulate enzyme preparation from Bacillus stearothermophilus synthesized 1,3-poly(glycerol phosphage) from CDPglycerol at an optimum pH of 8.0 and the reaction was stimulated by divalent cations. Km for CDPglycerol was 0.18 mM. The synthesis was inhibited by CMP, CDP, and CTP and by concentrations of CDP-glycerol above 0.49 mM. The reaction was irreversible, The product had an average chain length of 8 glycerol units. About two thirds of the polymers were synthesized in entirety while the ramainder were attached to some acceptor by their phosphate end. The enzome was able to synthesize only a limited amount of polymer.
MeSH terms
Calcium; Cations, Divalent; Cytosine Nucleotides; Depression, Chemical; Geobacillus stearothermophilus; Glycerophosphates; Hydrogen-Ion Concentration; Magnesium; Nucleoside Diphosphate Sugars; Polysaccharides, Bacterial
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