A comparison of canine normal hepatic alkaline phosphatase and variant alkaline phosphatase of serum and liver.
Clin Chim Acta, 1975/7/09;62(1):137-42.
PMID: 238771
Impact factor: 6.314
Abstract
The isoenzyme of alkaline phosphatase from normal liver, the corticosteroid induced isoenzyme of alkaline phosphatase from serum and liver and a hepatocellular variant isoenzyme of alkaline phosphatase induced by lymphosarcoma have been partially purified and their the present modification incorporates Polybrene into buffer to eliminate this heparin interference. The proposed method shown excellent agreement with a reference procedure based on clottable protein, and excellent day-to-day precision (C.V.3.5%). The present method is easily adaptable to semi-automated measurements.
MeSH terms
Adrenal Cortex Diseases; Adrenal Cortex Hormones; Alkaline Phosphatase; Animals; Carcinoma, Hepatocellular; Dogs; Drug Stability; Enzyme Induction; Hot Temperature; Hydrogen-Ion Concentration; Isoenzymes; Kinetics; Leucine; Liver; Liver Neoplasms; Lymphoma, Non-Hodgkin; Neoplasm Metastasis; Phenylalanine; Time Factors
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