Ternary solvents to investigate proteins at zub-zero temperatures.
Biochimie, 1975;57(1):43-8.
Travers F, Douzou P, Pederson T, Gunsalus IC
PMID: 238663
Impact factor: 4.372
Abstract
Mixtures of water, ethylene glycol and methanol in different volume ratios have been selected to carry out kinetics of enzyme reactions at sub-zero temperatures with the intention to reduce maximally the viscosity. Density, viscosity and dielectric constant values of these mixtures as a function of temperature are reported, as well as values of the protonic activity of several buffers under such conditions. A procedure to avoid or delay the eventual damaging effect of methanol on proteins is described.
MeSH terms
Acetates; Buffers; Cacodylic Acid; Cold Temperature; Densitometry; Enzymes; Ethylene Glycols; Freezing; Glycine; Hydrogen-Ion Concentration; Methanol; Phosphates; Proteins; Protons; Temperature; Tromethamine; Viscosity; Water
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