The substrate for cholesterol 7alpha-hydroxylase.
Biochim Biophys Acta, 1975/7/22;398(1):172-7.
Balasubramaniam S, Mitropoulos KA, Myant NB
PMID: 238645
Abstract
The rates of incorporation of (14C) cholesterol into cholesteryl esters and 5-cholestene-3beta,7alpha-diol (7alpha-hydroxycholesterol) by rat liver microsomes, measured under conditions in which esterification and 7alpha-hydroxylation are varied independently, indicated that cholesterol is the substrate for cholesterol 7alpha-hydroxylase. The specific activities of cholesteryl esters and 7alpha-hydroxycholeste ol in incubations of microsomes labelled with (14C)cholesterol in vitro or in vivo suggest that 7alpha-hydroxycholesterol and esterified cholesterol are not derived from the same pool of free cholesterol.
MeSH terms
Adenosine Triphosphate; Animals; Cholesterol 7-alpha-Hydroxylase; Coenzyme A; Esters; Kinetics; Microsomes, Liver; NADP; Oxidation-Reduction; Rats; Steroid Hydroxylases; Structure-Activity Relationship; Time Factors
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