Separation of mouse spleen haematopoietic cell fractions for transplantation purposes in lethally irradiated mice.
Physiol Bohemoslov, 1975;24(3):275-81.
Fiala J, Viktora L, Fricová V, Hrubá A, Pristoupil TI
PMID: 238253
Abstract
Mouse cells were fractioned by adherence chromatography on glass beads. The fractionation of spleen cells yielded 5 different fractions, whose transplantation effect was tested by the method of Till and McCulloch. The results of these transplantation tests showed that the chosen form of adherence chromatography was not sufficiently successful, since none of the resultant fractions contained solely colony-forming units (CFU) and in none of them were CFU present in an exeptionally high concentration.
MeSH terms
Animals; Antibody Formation; Bone Marrow; Bone Marrow Cells; Cattle; Cell Fractionation; Female; Graft vs Host Reaction; Hematopoietic Stem Cell Transplantation; Immune Sera; Mice; Mice, Inbred Strains; Radiation Effects; Radiation Injuries; Spleen; Transplantation, Homologous
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