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23667793
Target Identification by Diazirine Photo-Cross-linking and Click Chemistry.

Curr Protoc Chem Biol, 2009/12;1:55-73.

Mackinnon AL[1], Taunton J

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PMID: 23667793

Abstract
Target identification of biologically active small-molecules is often the rate-determining step in forward chemical genetics. Photo-affinity labeling (PAL) represents a useful biochemical strategy for target identification in complex protein mixtures. This unit describes the use of alkyl diazirine-based photo-affinity probes and Cu(I)-catalyzed click chemistry to covalently label and visualize the targets of biologically active small-molecules. A general method for affinity purification of probe-modified proteins, useful for identification of protein targets, is also described.

Keywords: affinity purification; click chemistry; diazirine; photo-affinity labeling; target identification

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