Action of rennin on kappa-casein.
J Dairy Sci, 1975/1;58(1):13-8.
PMID: 234484
Impact factor: 4.225
Abstract
The action of rennin on kappa-casein was studied as a function of time using several methods to measure activity. The first indication of rennin cleavage of kappa-casein is precipitability in .1 M acetate buffer at pH 5.2 and 5 C. A longer exposure to rennin is required to alter kappa-casein so that it forms a precipitate with calcium ions and loses its ability to stabilize alpha s-casein. The least sensitive indication of rennin activity is measurement of nitrogen soluble in 2% trichloroacetic acid. Electrophoresis experiments showed that these methods detect various stages in the conversion of kappa-casein para-kappa-casein.
MeSH terms
Calcium Chloride; Caseins; Centrifugation; Chemical Phenomena; Chemical Precipitation; Chemistry; Chymosin; Electrophoresis, Polyacrylamide Gel; Hydrogen-Ion Concentration; Nitrogen; Temperature; Time Factors; Trichloroacetic Acid
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