Studies on the differentiation of retinal pigmented epithelium cells in culture.
Invest Ophthalmol, 1975/2;14(2):108-13.
Chader GJ, Newsome DA, Bensinger RE, Fletcher RT
PMID: 234409
Abstract
Sephadex G-25 fractions of chick embryo extract affect the differentiation of cloned embryonic pigmented epithelial cells (PE) in culture. Both tyrosinase activity and amounts of melanin are markedly affected by the extract fractions. 5-Hydroxyindole decreased melanin accumulation in PE cells while alpha-methyl-p-tyrosine had no apparent effect on the process. Melanin disposal as well as biosynthesis may be important in controlling the accumulation of pigment in PE cells under culture conditions. Triiodothyronine greatly increased visible pigmentation and affected the morphology of cultured PE cells.
MeSH terms
Animals; Catechol Oxidase; Cell Differentiation; Cells, Cultured; Chick Embryo; Chromatography, Gel; Clone Cells; Dextrans; Embryo, Mammalian; Embryo, Nonmammalian; Epithelial Cells; Melanins; Pigmentation; Retina; Triiodothyronine; Tritium; Tyrosine; Tyrosine 3-Monooxygenase
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