[Analysis of sheep blood serum haptoglobin].
Biokhimiia, 1975/11-1975/12;40(6):1163-7.
Beĭsembaev RU, Dzhustupova RZh
PMID: 2330
Abstract
Three types of haptoglobin (Hp), differing in the number of bands of Hp--Hb complex on polyacrylamide gel electrophoregramm are found in sheep blood serum. HpA, HpB and HpC fractions included one, two-three and six-eight bands respectively. A modified procedure for the HpC isolation is described. Effects of urea, sodium dodecylsulpate, beta-mercaptoethanol, pH and maleinization on the behaviour of HpC under polyacrylamide gel electrophoresis is studied. The data obtained suggest that the HpC molecule consists of two subunits (of alpha and beta types), bound with S--S bounds in an alphabeta-dimer, which form a whole HpC molecule for the expense of non-covalent bonds.
MeSH terms
Animals; Binding Sites; Haptoglobins; Hydrogen-Ion Concentration; Macromolecular Substances; Mercaptoethanol; Protein Binding; Protein Conformation; Sheep; Sodium Dodecyl Sulfate; Urea
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