Chromosomal mobilization from a recA mutant of Pseudomonas putida.
Mol Gen Genet, 1979/10/02;176(1):151-4.
PMID: 232230
Abstract
A methyl methane sulfonate sensitive mutant of P. putida strain PpG1 is also extremely sensitive to UV-rays, compared to parent wild type cells. This mutant behaves typically as recombination less (recA) mutants of Escherichia coli and Pseudomonas aeruginosa, since as a recipient, it exhibits extremely low frequency of recombination following conjugational, transductional, and transformational gene transfer. Sex factor plasmids such as K-XYL or TOL can mobilize chromosomal genes equally well both from recA+ and recA801 donor cells, suggesting that host recombination functions are not necessary for mobilization of chromosomal genes by such plasmids.
MeSH terms
Bacterial Proteins; Chromosomes, Bacterial; Conjugation, Genetic; DNA Helicases; Plasmids; Pseudomonas; Recombination, Genetic; Transduction, Genetic; Transformation, Genetic
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