High-performance liquid chromatographic assay for acid and alkaline phosphatase in serum.
J Chromatogr, 1979/5/01;163(1):19-28.
Krstulovic AM, Hartwick RA, Brown PR
PMID: 231044
Abstract
High-performance liquid chromatography was used to assay serum acid and alkaline phosphatase. Samples were incubated with adenosine-5'-monophosphoric acid (AMP) in a buffer of required pH, 5'-nucleotidase was inhibited with Ni2+ ions, and the phosphatase activity was determined by measuring the concentration of the reaction product, adenosine. The analysis time, after the incubation is terminated, is short (7 min), and the assay is quantitative and reproducible. Complete separation of the reaction product from the substrate and the naturally occurring serum constituents and the high sensitivity of the ultraviolet detection system eliminate some of the problems commonly encountered in spectrophotometric assays.
MeSH terms
Acid Phosphatase; Adenosine; Alkaline Phosphatase; Chromatography, High Pressure Liquid; Hepatitis; Humans; Liver Cirrhosis; Nucleotidases; Reference Values
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