Determination of cholesterol and phospholipid concentrations in high-density lipoproteins by heparin-manganese chloride precipitation.
Clin Chim Acta, 1979/7/16;95(2):395-9.
PMID: 230919
Impact factor: 6.314
Abstract
Cholesterol as well as phospholipids in high-density lipoproteins (HDL) have been measured in 60 serum samples after heparin-manganese chloride precipitation of the apolipoprotein B containing lipoproteins. The values obtained correlated well with values from ultracentrifugal separation (slope 1.11 for cholesterol, 0.92 for phospholipids). Furthermore, there was a significant correlation between the low-density lipoprotein (LDL) cholesterol estimated by the formula of Friedewald et al. and the ultracentrifugally separated LDL cholesterol (slope 1.02). By the additional determination of HDL phospholipids with a precipitation procedure, further information about the "antiatherogenic" is easily available.
MeSH terms
Adult; Chemical Precipitation; Cholesterol; Heparin; Humans; Lipoproteins, HDL; Lipoproteins, LDL; Manganese; Middle Aged; Phospholipids; Ultracentrifugation
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