Experimental evidence for asymmetrical shielding of nucleosomal DNA by histones.
Nucleic Acids Res, 1979/12/20;7(8):2419-29.
PMID: 230466
Impact factor: 19.16
Abstract
Electron spin resonance study of Mn (II) binding to chromatin and derivatives, including core particles, shows that Mn (II) is a good probe for testing the overall electrostatic balance of the nucleoproteic complex as well as DNA accessibility. Experimental results are in good agreement with a recent model proposed (Mirzabekov A. D. and Rich A. (1979) Proc. Natl. Acad. Sci. USA 76, 1118-1121), for the core particle, in which an asymmetrical shielding of DNA by the protein core is assumed. Furthermore, it was found that the histone H1 hinders a number of charges on the linker DNA in a proportion equal to the net positive charge of the histone itself. This result is interpreted as due to a tighter interaction between the linker DNA and the core histones in the presence of histone H1.
MeSH terms
Animals; Binding Sites; Cattle; Chickens; Chromatin; DNA; Deoxyribonucleoproteins; Electron Spin Resonance Spectroscopy; Erythrocytes; Histones; Liver; Manganese; Nucleosomes; Rats; Thymus Gland
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