Specific chemical labeling of DNA fragments.
Nucleic Acids Res, 1979/11/24;7(6):1485-95.
Eshaghpour H, Söll D, Crothers DM
PMID: 228251
Impact factor: 19.16
Abstract
We describe a simple method for specific chemical labeling of DNA fragments at their 3'-termini. The procedure includes enzymatic addition of 4-thiouridine, followed by reaction in mild non-denaturing conditions with the highly reactive alpha-haloacetamido derivatives of several chemical labels. The attached reporter molecule can be removed by extended treatment with beta-mercaptoethanol. Among the potential applications of this labeling method is the study of specific protein-DNA interactions in solution.
MeSH terms
Chemical Phenomena; Chemistry; DNA; DNA Nucleotidyltransferases; Eosine Yellowish-(YS); Fluoresceins; Iodoacetamide; Methods; Naphthalenesulfonates; Thiouridine
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