Restoration of membrane excitability of squid giant axons by reagents activating tyrosine-tubulin ligase.
J Membr Biol, 1979/10/05;50(1):15-22.
PMID: 228042
Impact factor: 2.426
Abstract
Using squid giant axon, an experimental survey was performed on restoration of the membrane excitability which had been partially suppressed. Among reagents examined, a combination of 400 mM KF, 50 micrometers tyrosine, 1 mM ATP, 1 mM Mg ions and 2 micrometers cAMP was found to induce the restoration of the excitability to a large extent. Further addition of a small amount of either porcine brain microtubule proteins or the squid axoplasm was found to support complete restoration. The experiments suggest that tubulin-tyrosine ligase contained in the porcine brain microtubule protein fraction or the squid axoplasm maintains the coupling between cytoskeletal structures and the plasma membrane.
MeSH terms
Action Potentials; Adenosine Triphosphate; Animals; Axons; Cell Membrane; Cyclic AMP; Decapodiformes; Ligases; Magnesium; Membrane Potentials; Microtubules; Nucleotides; Tubulin; Tyrosine
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