Characterization of virus DNA synthesized in KB cells infected with two temperature-sensitive mutants of adenovirus type 5.
J Gen Virol, 1979/7;44(1):25-35.
Sussenbach JS, Kuijk MG, van Maarschalkerweerd MW
PMID: 227996
Impact factor: 5.141
Abstract
KB cells were infected with H5ts36 or H5ts125, two adenovirus type 5 (Ad5) mutants with a temperature-sensitive synthesis of virus DNA. Infection was started at the nonpermissive temperature and at 16 h p.i. the temperature was shifted down to the permissive temperature. Shortly after the shift-down H5ts125-infected cells showed an accumulation of purely single-stranded DNA of virus origin, which was not observed in H5ts36-infected cells. This single-stranded DNA has been characterized by hybridization and sedimentation analysis. It was found that the single-stranded DNA was derived from both complementary strands and consisted of short fragments. The observation that the single-stranded DNA accumulates in H5ts125-infected cells under conditions in which the amount of DNA binding protein is reduced, suggests that the DNA-binding protein is not only involved in initiation, but also in elongation of nascent strands.
MeSH terms
Adenoviruses, Human; Carcinoma; Cell Line; DNA Helicases; DNA Replication; DNA, Single-Stranded; DNA, Viral; Humans; Mouth Neoplasms; Mutation; Temperature; Viral Proteins
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