[A new method for determination of ATPase activity in tissue homogenates].
Boll Soc Ital Biol Sper, 1978/12/30;54(24):2577-82.
Martino G, Testa M, Vicinanza P, Habetswallner E
PMID: 227435
Abstract
Authors describe a new method to determine Mg2+ and Na+K+ATPase activity on extremely small samples of tissue homogenates. They use specific inhibitors to discern between the two activities and a sensitive colorimetric method to dose Pi released from ATP, Lower limit of sensitivity is about 0,0100 micromoles of Pi/mg proteins/hour.
MeSH terms
Adenosine Triphosphatases; Animals; Brain; Clinical Enzyme Tests; Colorimetry; Humans; Kidney Glomerulus; Liver; Muscles; Phosphates; Rats; Sodium-Potassium-Exchanging ATPase
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