Differential sensitivity of gene expression in vitro to inhibitors of DNA gyrase.
Proc Natl Acad Sci U S A, 1979/7;76(7):3304-8.
Yang HL, Heller K, Gellert M, Zubay G
PMID: 226966
Impact factor: 12.779
Abstract
We have used the antibiotics coumermycin A1, novobiocin, and oxolinic acid, which are specific inhibitors of DNA gyrase, to study the coupled transcription and translation of several bacterial and plasmid genes in a DNA-directed cell-free system. The expression of different genes is reduced to different extents by inhibition of DNA gyrase activity. Among the genes tested, the lac operon, an rRNA gene, and the colicin gene of colicin E1 plasmid were found to be most sensitive, while the trp operon and some other genes in colicin E1 plasmid were relatively unaffected by the inhibitors. These results, together with earlier work on the transcription of circular DNA templates, indicate that DNA supercoiling can significantly enhance transcription from certain promoters.
MeSH terms
Anti-Bacterial Agents; Coumarins; DNA; DNA, Circular; DNA, Superhelical; Genes; Novobiocin; Oxolinic Acid; Plasmids; Protein Biosynthesis; Pyrroles; Topoisomerase II Inhibitors; Transcription, Genetic
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