[A simple micromethod for sphingomyelinase determination (author's transl)].
Clin Chim Acta, 1979/8/01;95(3):447-51.
Maziere JC, Maziere C, Polonovski J
PMID: 226287
Impact factor: 6.314
Abstract
A rapid and sensitive micromethod for routine determination of sphingomyelinase activity of leukocytes, fibroblasts and hair roots is described. This method makes use of the natural radioactive substrate of the enzyme. The product of the reaction is isolated by direct application of the incubation mixture on silica gel plates followed by ascending chromatography. The sensitivity of the method allows sphingomyelinase determination in less than 0.5 microgram of fibroblast proteins.
MeSH terms
Carbon Radioisotopes; Fibroblasts; Hair; Humans; Leukocytes; Methods; Phosphoric Diester Hydrolases; Sphingomyelin Phosphodiesterase
More resources
EndNote: Download