[Study of sarcoplasmic reticulum Ca-ATPase by the paramagnetic label--paramagnetic probe method].
Biofizika, 1979/9-1979/10;24(5):839-42.
Kotel'nikova RA, Tat'ianenko LV, Kulikov AV, Kotel'nikov AI, Mel'nikov AV
PMID: 226172
Abstract
ESR spectra of nitroxyl derivative labelling with parachlormercury benzoate bound with SH-groups of Ca, Mg-dependent ATPase in the presence of Mn2+ ions are studied. It has been concluded from the saturation curves of ESR spectra that Mn2+ is localized at the distance approximately 40 A from the SH-group of the enzyme active centre. Platinum compound (K2PtCl4) changes the spectrum of ESR 1 due to the displacement of the label from the enzyme SH-groups and disintegration of the sarcoplasmic reticulum structure. Palladium compound (Pd(NH3)4Cl2) produced no effect on the ESR 1 spectrum.
MeSH terms
Animals; Binding Sites; Calcium-Transporting ATPases; Chemical Phenomena; Chemistry; Chloromercuribenzoates; Electron Spin Resonance Spectroscopy; Magnesium; Manganese; Palladium; Platinum; Rabbits; Sarcoplasmic Reticulum
More resources
EndNote: Download