Regulation of sterol synthesis in human lymphocytes: evidence for post-transcriptional control by low density lipoprotein.
Biochim Biophys Acta, 1979/8/30;574(2):361-5.
Krone W, Betteridge DJ, Galton DJ
PMID: 226155
Abstract
The effect of cordycepin (3'-deoxyadenosine), an inhibitor of messenger RNA synthesis, on the induction of 3-hydroxy-3-methylglutaryl coenzyme A reductase mediated by lipid-depleted serum was studied in isolated human lymphocytes. 50 micrograms/ml cordycepin, although inhibiting messenger RNA synthesis by more than 50%, had no inhibitory effect on the two and four-fold induction of hydroxymethylglutaryl-CoA reductase when cells were incubated in a medium containing lipid-depleted serum for 8 and 16 h, respectively. This result suggests that newly synthesises messenger RNA is not required for the effect of lipid-depleted serum on the induction of hydroxymethylglutaryl-CoA reductase in human lymphocytes.
MeSH terms
Deoxyadenosines; Humans; Hydroxymethylglutaryl CoA Reductases; In Vitro Techniques; Lipoproteins, LDL; Lymphocytes; RNA, Messenger; Sterols
More resources
EndNote: Download