De novo sequencing and analysis of the termite mushroom (Termitomyces albuminosus) transcriptome to discover putative genes involved in bioactive component biosynthesis.
J Biosci Bioeng, 2012/8;114(2):228-31.
Yang F[1], Xu B, Zhao S, Li J, Yang Y, Tang X, Wang F, Peng M, Huang Z
Affiliations
PMID: 22608552DOI: 10.1016/j.jbiosc.2012.03.009
Impact factor: 3.185
Abstract
A one-eighth 454 sequencing run produced 82,386 high-quality reads. De novo assembly generated 6494 unique sequences. Based on the bioinformatic analysis, we found many the known enzymes involved in the biosynthesis of triterpene saponin in Termitomyces albuminosus, including 6 cytochrome P450 and 22 glycosyltransferase unique genes.
MeSH terms
Cytochrome P-450 Enzyme System; Expressed Sequence Tags; Ginsenosides; Glycosyltransferases; Saponins; Sequence Analysis, DNA; Termitomyces; Transcriptome; Triterpenes
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