Simian virus 40-transformed cells express new species of proteins precipitable by anti-simian virus 40 tumor serum.
J Virol, 1979/8;31(2):472-83.
Kress M, May E, Cassingena R, May P
PMID: 225566
Impact factor: 6.549
Abstract
In addition to the virus-coded large-T and small-t antigens, two new classes of proteins were immunoprecipitated by anti-simian virus 40 (SV40) tumor serum from extracts of various SV40-transformed cell lines. These were as follows: (i) proteins (termed "super-T proteins") with an Mr higher than that of large-T antigen (86,000), which were found in many SV40-transformed cell lines derived from mouse and rat cells (super-T proteins and large-T antigen appeared to have closely related structures as judged by the Chromobead elution patterns of their methionine-labeled tryptic peptides); (ii) proteins (termed "55K proteins") with an Mr ranging from 50,000 to 60,000, which were present in all SV40-transformed cell lines examined so far, including those obtained by chromosome-mediated gene transfer. The 55K proteins were not structurally related to large-T antigens, as judged by the Chromobead elution patterns of their methionine-labeled tryptic peptides. Our data are compatible with the assumption that the 55K proteins are largely or totally cell coded.
MeSH terms
Animals; Antigens, Neoplasm; Antigens, Viral; Cell Line; Cell Transformation, Neoplastic; Cell Transformation, Viral; Cricetinae; Haplorhini; Humans; Immune Sera; Kidney; Lung; Mice; Neoplasms, Experimental; Rats; Simian virus 40; Skin; Viral Proteins
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