Inhibition of 80S initiation complex formation by infection with poliovirus.
J Gen Virol, 1979/5;43(2):441-5.
PMID: 225427
Impact factor: 5.141
Abstract
Anisomycin has been shown to stabilize ribosome initiation complexes containing messenger RNA and met-tRNAf met to high salt conditions. Extracts from HeLa cells treated with 5 X 10(-7) M-anisomycin for 15 min accumulate 80S initiation complexes which can be detected by their absorbance in sucrose gradients. Poliovirus-infected cells fail to form the 80S initiation complex early after infection, when inhibition of host cell protein synthesis occurs. These complexes re-form later in infection after virus RNA is synthesized. No re-formation occurs in the absence of virus replication. Thus, the step in protein synthesis inhibited by poliovirus precedes the entry of components into the 80S initiation complex.
MeSH terms
Anisomycin; HeLa Cells; Humans; Methionine; Neoplasm Proteins; Peptide Chain Initiation, Translational; Poliovirus; RNA, Messenger; RNA, Neoplasm; RNA, Transfer, Amino Acyl; Ribosomes; Virus Replication
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