[Estimation of methods for isolation of 3':5'-AMP-dependent protein kinases from animal tissues].
Ukr Biokhim Zh (1978), 1979/5-1979/6;51(3):297-304.
PMID: 223263
Abstract
Different approaches are described to isolation and purification of soluble and membrane-bound 3':5'-AMP-dependent protein kinases from different tissues and intracellular structures. Main stages of the enzyme isolation: homogenization of tissues, acidic precipitation, fractionation by ammonium sulphate, chromatography on ion-exchangers and gel filtration, are characterized.
MeSH terms
Animals; Cyclic AMP; Enzyme Activation; Evaluation Studies as Topic; Membranes; Methods; Protein Kinases
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