Isolation and culture of endothelial cells from the lungs of small animals.
Am Rev Respir Dis, 1979/6;119(6):853-68.
Habliston DL, Whitaker C, Hart MA, Ryan US, Ryan JW
PMID: 222181
Abstract
Techniques are descirbed for the isolation and culture of endothelial cells from the lungs of small animals. The cells are collected by retrograde perfusion of blood-free lungs with buffered saline containing collagenase. The cells are characterized by light microscopy, electron microscopy of thin sections and surface replicas, and by the presence of angiotensin-converting enzyme (ACE). ACE was assayed using 3H-benzoyl-Phe-Ala-Pro as substrate and was localized by indirect immunofluorescence using guinea pig endothelial cells incubated with rabbit antibodies to guinea pig lung ACE followed by goat anti-rabbit globulins conjugated to fluorescein. Thus, endothelial cultures can be established using small animals commonly employed in studies of pulmonary processing of vasoactive substances.
MeSH terms
Animals; Cell Separation; Cells, Cultured; Chromosomes; Cytological Techniques; Endothelium; Female; Fluorescent Antibody Technique; Guinea Pigs; Lung; Male; Microbial Collagenase; Microscopy, Electron; Peptidyl-Dipeptidase A; Perfusion; Rabbits; Rats
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