Solubilized and insolubilized bone morphogenetic protein.
Proc Natl Acad Sci U S A, 1979/4;76(4):1828-32.
Urist MR, Mikulski A, Lietze A
PMID: 221908
Impact factor: 12.779
Abstract
A bone morphogenetic protein (BMP) obtained in solution by digestion of demineralized rabbit cortical bone matrix with bacterial collagenase retains its biologically active conformation in a neutral salt/ethylene glycol mixture. BMP may be insolubilized by coprecipitation with calcium phosphate and resolubilized by chemical extraction with a neutral salt in the same solvent mixture. Upon concanavalin A-Sepharose chromatography, BMP is bound by hydrophobic interaction and carbohydrate recognition and is recovered by elution with either alpha-methyl mannoside or ethylene glycol solvent mixture. Implants of both eluates and the extracts of the coprecipitate in double-walled diffusion chambers induce transmembrane bone morphogenesis. BMP is not species specific; rabbit BMP induces new bone formation in the rat. The present observations indicate that BMP is a glycoprotein.
MeSH terms
Animals; Biological Assay; Bone and Bones; Microbial Collagenase; Protein Conformation; Proteins; Rabbits; Solubility
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