Differential synthesis of lens proteins in the presence of m7G(5')pppG and cleavage product m7GMP in an embryonic chick lens cell lysate.
Mol Biol Rep, 1979/2/15;4(4):233-6.
PMID: 220521
Impact factor: 2.742
Abstract
The effect of m7GMP release from m7GMP-containing mRNA cap sequence m7GpppG by the embryonic chick lens m7GpppN-pyrophosphatase activity on the synthesis of lens proteins was examined in a newly developed homologous translation system derived from 15-day embryonic chick lenses. The synthesis of total lens polypeptides and delta-crystallin polypeptides, the major translation product, was inhibited 84% and 88%, respectively, by 0.5 mM m7GpppG; m7GMP (0.5 mM) inhibited total synthesis by 63% but was 33% less inhibitory toward delta-crystallin synthesis; GpppG and GMP were not inhibitors, m7GpppG inhibited met-tRNAfmet incorporation into 80S initiation complexes.
MeSH terms
Animals; Chick Embryo; Crystallins; Guanine Nucleotides; Guanosine Monophosphate; Lens, Crystalline; Peptide Chain Elongation, Translational; Peptide Chain Initiation, Translational; RNA, Messenger; RNA, Transfer
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