Extraction of soluble antigens of Epstein-Barr virus, Herpesvirus salmirl, and Herpesvirus ateles with the use of glycine.
J Natl Cancer Inst, 1979/5;62(5):1173-5.
Ablashi DV, Easton JM, Armstrong GR, Bengali Z
PMID: 220447
Impact factor: 11.816
Abstract
For extraction of soluble antigen from cells infected with Epstein-Barr virus, Herpesvirus salmirl, and H. ateles, 0.1 M glycine (pH 9.5) was used. This method yielded increased amounts of the antigen containing much less cell debris. Lymphoblastoid cells infected with Epstein-Barr virus could maintain up to 50% viability after the extraction procedure. These cells could be used again after an appropriate interval in culture. The usefulness of this technique is discussed.
MeSH terms
Antigens, Viral; Glycine; Herpesviridae; Herpesvirus 2, Saimiriine; Herpesvirus 4, Human; Immunologic Techniques; Microbiological Techniques
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