Arrangement of glucose residues in the lipid-linked oligosaccharide precursor of asparaginyl oligosaccharides.
J Biol Chem, 1979/6/10;254(11):4554-9.
Liu T, Stetson B, Turco SJ, Hubbard SC, Robbins PW
PMID: 220251
Abstract
The lipid-linked oligosaccharide synthesized in vitro, in the presence of 1.0 microM UDP-[3H]Glc, GDP-[14C]Man, and UDP-GlcNAc has been isolated and the structure of the oligosaccharide has been analyzed. The oligosaccharide contains 2 N-acetylglucosamine, 9 mannose, and 3 glucose residues. The N-acetylglucosamine residues are located at the reducing terminus. The 3 glucose residues are arranged in a linear order at one of the nonreducing termini in the sequence Glc 1,2--Glc 1,3--Glc--(Man)9 (GlcNAc)2. The structural analysis was made possible largely by the availability of glucosidase preparations of fungal anad microsomal origin which remove glucose residues from the oligosaccharide without releasing mannose residues.
MeSH terms
Asparagine; Cell Line; Glucose; Mannosidases; Methylation; Oligosaccharides; Oxidation-Reduction; Periodic Acid; Sindbis Virus
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