Spontaneous and induced cytotoxic properties of human adherent mononuclear cells: killing of non-sensitized and antibody-coated non-erythroid cells.
Immunology, 1979/2;36(2):221-8.
Horwitz DA, Kight N, Temple A, Allison AC
PMID: 220181
Impact factor: 7.215
Abstract
A method is described which demonstrates that human adherent mononuclear cells have a strong capacity to kill human nucleated target cells. Cytotoxicity was measured by the release of 51chromium from two established cell lines. Freshly prepared monolayers killed both non-sensitized and anti-body coated K562 and CLA-4 target cells. These spontaneous events decreased after short term culture. A second peak of cytotoxic activity was induced by activating the effector cells with endotoxin-treated serum or phytohaemagglutinin. Cytotoxicity was inhibited by silica particles. Studies with alpha-naphthyl esterase showed that 95% of freshly prepared cells and 99% of monolayers cultured for 68 h were monocytes. These studies suggest that adherent monocytes are the predominant cytotoxic effector cell although a contributing effect by the small numbers of contaminating lymphocytes can not be excluded. Evaluation of monocyte cytotoxicity should have a useful role in clinical investigation.
MeSH terms
Antibody-Dependent Cell Cytotoxicity; Cell Adhesion; Cell Line; Cytotoxicity, Immunologic; Endotoxins; Humans; Monocytes; Neoplasms, Experimental; Phytohemagglutinins; Silicon Dioxide
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