[Mechanism of catalysis by o-diphenol oxidase].
Biokhimiia, 1979/4;44(4):741-7.
Bresler SE, Kazbekov EN, Sukhodolova AT, Shadrin VN
PMID: 219916
Abstract
The reaction of terminal oxidation of the substrate (catechol) by molecular oxygen catalyzed by o-diphenoloxidase (o-diphenol: oxygen oxydoreductase; EC 1.10.3.1) is found to occur via a free radical mechanism. The copper of the active center changes its valency during the reaction. The spectra of substrate radicals and of the Cu2+ ions were registered by means of a high sensitivity ESR-spectrometer and their concentrations were determined.
MeSH terms
Binding Sites; Catalysis; Catechol Oxidase; Chemical Phenomena; Chemistry; Electron Spin Resonance Spectroscopy; Free Radicals
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