Single strand DNA binding of simian virus 40 tumor antigen.
J Biol Chem, 1979/4/25;254(8):3100-4.
Spillman T, Giacherio D, Hager LP
PMID: 218962
Abstract
Simian virus 40 T antigen binds to both single and double strand DNA. The single and double strand DNA binding activity of crude T antigen preparations was evaluated by chromatography of the antigen on DNA-cellulose columns. Crude T antigen was retained on both native and denatured DNA-cellulos columns and was eluted from both columns under similar conditions. The interaction of highly purified T antigen with single and double strand DNA was evaluated by competition experiments using a DNA filter binding assay. These experiments showed that T antigen binds preferentially to single strand calf thymus DNA by more than an order of magnitude when compared to double strand calf thymus DNA.
MeSH terms
Antigens, Neoplasm; Antigens, Viral; Binding, Competitive; DNA, Single-Stranded; Kinetics; Molecular Weight; Protein Binding; Simian virus 40
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