A simple large-scale method for separating closed circular form DNA by gel electrophoresis.
Acta Med Okayama, 1978/12;32(6):379-85.
Tanabe N, Hidaka H, Watanabe S, Oda T
PMID: 218425
Impact factor: 0.744
Abstract
The covalently closed form of circular duplex SV40 DNA was separated from the open and linear form of SV40 DNA by agarose gel electrophoresis in a large-scale gel system. The closed circular DNA was recovered from agarose gels by re-electrophoresing the gel slices. The recovery of DNA was about 70%. Electron microscopic analysis showed that the recovered DNA did not have doube- or single-stranded breaks. The recovered DNA can be used without further purification for electron microscopy, as a substrate for experiments using restriction endonuclease and as a template for in vitro RNA synthesis.
MeSH terms
Animals; Cell Line; Chlorocebus aethiops; DNA, Circular; DNA, Viral; Electrophoresis; Electrophoresis, Agar Gel; Haplorhini; Kidney; Molecular Weight; Simian virus 40
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