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218224
Transfection of human lymphoblastoid cells with herpes simplex viral DNA.

Proc Natl Acad Sci U S A, 1979/2;76(2):949-53.

Miller G, Wertheim P, Wilson G, Robinson J, Geelen JL, van der Noordaa J, van der Eb AJ

PMID: 218224

Impact factor: 12.779

Abstract
The "calcium/dimethyl sulfoxide shock" method of transfection was adapted for use in human lymphoid cell cultures. One microgram of herpes simplex virus type 1 DNA regularly initiated virus replication in four lymphoblastoid cell lines. Per 10(5) cells exposed to 1 microgram of DNA, 0.5--5 cells formed an infectious center. The minimal infective dose of DNA was approximately 500 ng.
MeSH terms
Cell Line; DNA, Viral; Humans; Lymphocytes; Simplexvirus; Transfection; Virus Replication
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