Covalent cross-linking of photosensitive phospholipids to human serum high density apolipoproteins (apoHDL).
Hoppe Seylers Z Physiol Chem, 1979/2;360(2):197-206.
PMID: 217815
Abstract
Human serum high density apolipoproteins were reassociated with three different lecithin species substituted with radioactively labelled photosensitive azido fatty acids, bis([3H]-16-azidopalmitoyl)-, bis([3H]12-azidooleoyl)- and bis([3H]18-azidolinoleoyl)glycerophosphocholine. The lipoprotein particles were reconstituted from a mixture of azido-labelled phosphatidylcholine and non-labelled dioleoylglycerophosphocholine (1:9). Excess lipid was separated from the homogeneous particles by Bio-Gel A-5m. The molecular weight, stoichiometry, fluorescence and circular dichroism of the reconstituted particles were determined before and after photoactivation with covalent cross-linking of the phospholipids with the apoproteins. The physical parameters of the reconstituted lipoproteins remained unperturbed by the cross-linking reaction between the generated nitrenes and apolipoprotein A-I and A-II. Thus the hydrophobic interactions of the phospholipid molecules with the apoproteins have been proved for the first time by a chemical method.
MeSH terms
Apolipoproteins; Azides; Circular Dichroism; Humans; Lipoproteins, HDL; Phosphatidylcholines; Phospholipids; Photochemistry; Protein Binding; Protein Conformation; Spectrophotometry, Ultraviolet
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