Synthesis of rabbit beta-globin in cultured monkey kidney cells following infection with a SV40 beta-globin recombinant genome.
Nature, 1979/1/11;277(5692):108-14.
Mulligan RC, Howard BH, Berg P
PMID: 215915
Impact factor: 69.504
Abstract
Rabbit beta-globin complementary DNA (cDNA) has been inserted into SV40 DNA in place of the gene coding for the virus' major capsid protein, VP1. The recombinant genome, SVGT5-RabetaG, multiplies efficiently in CV1 monkey kidney cell cultures and is transcribed to yield cytoplasmic, polyadenylated hybrid mRNAs containing the beta-globin coding sequence. Cells propagating SVGT5-RabetaG produce substantial quantities of rabbit beta-globin polypeptide.
MeSH terms
Animals; Cell Line; DNA Restriction Enzymes; DNA, Recombinant; Genes, Viral; Globins; Methods; Nucleic Acid Precursors; RNA, Messenger; Rabbits; Simian virus 40; Viral Proteins
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