Polymorphism of apolipoprotein E. I. Methodological aspects and diagnosis of hyperlipoproteinemia type III without ultracentrifugation.
Clin Genet, 1978/12;14(6):351-8.
Utermann G, Albrecht G, Steinmetz A
PMID: 215358
Impact factor: 4.296
Abstract
Two methods for phenotyping apolipoprotein E are compared. One is based on preparation of VLDL by conventional ultracentrifugation, whereas the other uses heparin/Mg precipitation of VLDG. In principle, the same results were obtained by both methods. However, the group limits for the three different phenotypes Apo E-N, Apo E-ND and Apo E-D were slightly different by the two methods. Phenotype Apo E-D - the phenotype characterizing type III dyslipoproteinemia - is clearly definable by both methods. Hence the precipitation-I.E.F. method for Apo E phenotyping provides a simple tool for genetic and population genetic studies and also for the routine diagnosis of hyperlipoproteinemia type III, based on the only specific marker known for this disease.
MeSH terms
Apolipoproteins; Chemical Precipitation; Densitometry; Evaluation Studies as Topic; Heparin; Humans; Hyperlipidemias; Isoelectric Focusing; Lipoproteins, VLDL; Magnesium; Methods; Phenotype; Polymorphism, Genetic; Ultracentrifugation
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