Cyclic nucleotide levels in mouse mammary epithelial cells during growth arrest and growth initiation in culture.
J Natl Cancer Inst, 1977/7;59(1):145-9.
Howard EF, Scott DF, Manter JO
PMID: 195065
Impact factor: 11.816
Abstract
Intracellular levels of cyclic AMP (cAMP) and cyclic GMP (cGMP) were measured in high and low tumorigenic mouse mammary epithelial cells during growth arrest in 1% fetal bovine serum and during the first 60 minutes after serum stimulation of cell proliferation in arrested cultures. Stationary MCG-T14 cells, which are highly tumorigenic and grow to high densities in 1% serum, exhibited lower levels of cAMP, higher levels of cGMP, and a lower ratio of cAMP to cGMP than quiescent MCG-V14 cells, which have low tumorigenicity and achieve low cell densities in 1% serum. Within 5-10 minutes after cell growth was initiated in arrested cultures by the addition of serum, both cell lines responded with a fourfold to fivefold increase in cGMP and a concomitant 50% decrease in cAMP. MCG-T14 cells exhibited the highest intracellular levels of cGMP and the lowest cAMP to cGMP ratio within 10 minutes after serum addition.
MeSH terms
Animals; Cell Division; Cell Line; Culture Media; Cyclic AMP; Cyclic GMP; Epithelial Cells; Epithelium; Mammary Neoplasms, Experimental
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