Preparation, characterization, and anti-tumor property of podophyllotoxin-loaded solid lipid nanoparticles.
Nanotechnology, 2009/2/04;20(5):055702.
Zhu RR[1], Qin LL, Wang M, Wu SM, Wang SL, Zhang R, Liu ZX, Sun XY, Yao SD
Affiliations
PMID: 19417361DOI: 10.1088/0957-4484/20/5/055702
Impact factor: 3.953
Abstract
In an effort to develop an alternative formulation of podophyllotoxin suitable for drug release and delivery, podophyllotoxin-loaded solid lipid nanoparticles (PPT-SLNs) were constructed, characterized and examined for in vitro cytotoxicity and tumor inhibition. The SLNs were prepared by using a solvent emulsification-evaporation method, and their size was around 50 nm. TEM detection showed that the SLNs were homogeneous and spherical in shape, and differential scanning calorimetry (DSC) measurement revealed a new conformation of PPT-SLNs. An in vitro drug release study showed that PPT was released from the SLNs in a slow but time-dependent manner. Furthermore, the treatment of 293T and HeLa cells with PPT-SLNs demonstrated that PPT-SLNs were less toxic to normal cells and more effective in anti-tumor potency compared with unconjugated PPT. A colony forming efficiency assay showed an effective long-term cancer growth suppression of PPT-SLNs; in addition, they can also enhance the apoptotic and cellular uptake processes on tumor cells compared with PPT. These results collectively demonstrated that this SLN formulation has a potential application as an alternative delivery system for anti-tumor drugs.
MeSH terms
Antineoplastic Agents; Apoptosis; Crystallization; Drug Carriers; Drug Compounding; HeLa Cells; Humans; Lipids; Macromolecular Substances; Materials Testing; Molecular Conformation; Nanomedicine; Nanostructures; Particle Size; Podophyllotoxin; Surface Properties
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