Cholesterol determination in high-density lipoproteins separated by three different methods.
Clin Chem, 1977/5;23(5):882-4.
Lopes-Virella MF, Stone P, Ellis S, Colwell JA
PMID: 192488
Impact factor: 12.167
Abstract
We describe a simplified method for measuring high-density lipoprotein cholesterol in serum after very-low- and low-density lipoproteins have been precipitated from the specimen with sodium phosphotungstate and Mg2+. Values so obtained correlate well with values obtained with the heparin-Mn2+ precipitation technique (r = 0.95, CV less than 5% in 66% of the subjects studied and between 5 and 10% in the remaining ones) or by ultracentrifugal separation (r = 0.82, CV less than 5% in 80% of the subjects studied and between 5 and 10% in the remaining ones). Our precipitation technique is more appropriate for routine clinical laboratory use.
MeSH terms
Adult; Chemical Precipitation; Cholesterol; Heparin; Humans; Lipoproteins, HDL; Magnesium; Manganese; Methods; Phosphotungstic Acid; Triglycerides; Ultracentrifugation
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