Autoradiographic localization of prolactin receptors in 7,12-dimethylbnez[a]anthracene-induced rat mammary carcinoma.
J Natl Cancer Inst, 1977/4;58(4):1173-5.
PMID: 191627
Impact factor: 11.816
Abstract
Prolactin receptors were localized by autoradiography in 7,12-dimethylbenz[a]anthracene (DMBA)-induced mammary tumors from rats by incubation of tumor slices with [125I]ovine prolactin. Specific prolactin binding was confined to tumor cells, and nonspecific binding was present in alveolar spaces and connective tissue. In some tumors, all cells contained receptors; in others, up to one-half the cells remained unlabeled. These results suggested that variation in receptor content in DMBA-induced mammary tumors may be caused by two distinct populations of cells--one which contains receptors and another which possesses very few receptor sites or none at all.
Keywords: Animals, Laboratory; Biology; Breast Cancer; Cancer; Clinical Research; Diseases; Endocrine System; Hormone Receptors; Hormones; In Vitro; Membrane Proteins; Neoplasms; Physiology; Pituitary Hormones; Prolactin; Research Methodology
MeSH terms
9,10-Dimethyl-1,2-benzanthracene; Animals; Autoradiography; Benz(a)Anthracenes; Female; Mammary Neoplasms, Experimental; Prolactin; Rats; Receptors, Cell Surface
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